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Article Navigation >  Green Energy&Environment  > 2023  > Accepted Manuscript
Kai Wang, Changsheng Su, Haoran Bi, Changwei Zhang, Di Cai, Yanhiu Liu, Meng Wang, Biqiang Chen, Jens Nielsen, Zihe Liu, Tianwei Tan. The transition from 2G to 3G-feedstocks enabled efficient production of fuels and chemicals. Green Energy&Environment. doi: 10.1016/j.gee.2023.11.004
Citation: Kai Wang, Changsheng Su, Haoran Bi, Changwei Zhang, Di Cai, Yanhiu Liu, Meng Wang, Biqiang Chen, Jens Nielsen, Zihe Liu, Tianwei Tan. The transition from 2G to 3G-feedstocks enabled efficient production of fuels and chemicals. Green Energy&Environment. doi: 10.1016/j.gee.2023.11.004
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The transition from 2G to 3G-feedstocks enabled efficient production of fuels and chemicals

doi: 10.1016/j.gee.2023.11.004

  • 1 College of Life Science and Technology, Beijing University of Chemical Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR China;

  • 2 National Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, 100029 Beijing, PR China;

  • 3 Department of Life Sciences, Chalmers University of Technology, SE41296 Gothenburg, Sweden

Funds:

This work was supported by the National Key R&D Program of China [2021YFC2103500], National Natural Science Foundation of China (22211530047), Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [grant numbers: TSBICIP-KJGG-009], the Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology.

  • Received date 25 August 2023, Accepted date 20 November 2023, RevRecd date 12 November 2023, Available online 23 November 2023
    Abstract
  • For decades micoorganisms have been engineered for the utilization of lignocellulose-based second-generation (2G) feedstocks, but with the concerns of increased levels of atmospheric CO2 causing global warming there is an emergent need to transition from the utilization of 2G feedstocks to third-generation (3G) feedstocks such as CO2 and its derivatives. Here, we established a yeast platform that is capable of simultaneously converting 2G and 3G feedstocks into bulk and value- added chemicals. We demonstrated that by adopting 3G substrates such as CO2 and formate, the conversion of 2G feedstocks could be substantially improved. Specifically, formate could provide reducing power and energy for xylose conversion into valuable chemicals. Simultaneously, it can form a concentrated CO2 pool inside the cell, providing thermodynamically and kinetically favoured amounts of precursors for CO2 fixation pathways, e.g. the Calvin–Benson–Bassham (CBB) cycle. Furthermore, we demonstrated that formate could directly be utilized as a carbon source by yeast to synthesize endogenous amino acids. The engineered strain achieved a one-carbon (C1) assimilation efficiency of 9.2%, which was the highest efficiency observed in the co-utilization of 2G and 3G feedstocks. We applied this strategy for productions of both bulk and value-added chemicals, including ethanol, free fatty acids (FFAs), and longifolene, resulting in yield enhancements of 18.4%, 49.0%, and ~100%, respectively. The strategy demonstrated here for co-utilization of 2G and 3G feedstocks sheds lights on both basic and applied research for the up-coming establishment of 3G biorefineries.

     

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