Volume 9 Issue 11
Nov.  2024
Turn off MathJax
Article Contents
Article Navigation >  Green Energy&Environment  > 2024  >  9(11): 1759-1770
Kai Wang, Changsheng Su, Haoran Bi, Changwei Zhang, Di Cai, Yanhui Liu, Meng Wang, Biqiang Chen, Jens Nielsen, Zihe Liu, Tianwei Tan. The transition from 2G to 3G-feedstocks enabled efficient production of fuels and chemicals. Green Energy&Environment, 2024, 9(11): 1759-1770. doi: 10.1016/j.gee.2023.11.004
Citation: Kai Wang, Changsheng Su, Haoran Bi, Changwei Zhang, Di Cai, Yanhui Liu, Meng Wang, Biqiang Chen, Jens Nielsen, Zihe Liu, Tianwei Tan. The transition from 2G to 3G-feedstocks enabled efficient production of fuels and chemicals. Green Energy&Environment, 2024, 9(11): 1759-1770. doi: 10.1016/j.gee.2023.11.004
shu

The transition from 2G to 3G-feedstocks enabled efficient production of fuels and chemicals

doi: 10.1016/j.gee.2023.11.004

  • a. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, No. 15 North 3rd Ring Rd East, Beijing, 100029, China;

  • b. National Energy R&D Center for Biorefnery, Beijing Key Lab of Bioprocess, Beijing University of Chemical Technology, No. 15 North 3rd Ring Rd East, Beijing, 100029, China;

  • c. Department of Life Sciences, Chalmers University of Technology, Gothenburg, SE41296, Sweden

Funds:

This work was supported by the National Key R&D Program of China [2021YFC2103500], National Natural Science Foundation of China (22211530047), Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project [grant numbers: TSBICIP-KJGG-009], the Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology.

  • Received date 25 August 2023, Accepted date 20 November 2023, RevRecd date 12 November 2023, Publish date 22 November 2023,
    Abstract
  • For decades micoorganisms have been engineered for the utilization of lignocellulose-based second-generation (2G) feedstocks, but with the concerns of increased levels of atmospheric CO2 causing global warming there is an emergent need to transition from the utilization of 2G feedstocks to third-generation (3G) feedstocks such as CO2 and its derivatives. Here, we established a yeast platform that is capable of simultaneously converting 2G and 3G feedstocks into bulk and value-added chemicals. We demonstrated that by adopting 3G substrates such as CO2 and formate, the conversion of 2G feedstocks could be substantially improved. Specifically, formate could provide reducing power and energy for xylose conversion into valuable chemicals. Simultaneously, it can form a concentrated CO2 pool inside the cell, providing thermodynamically and kinetically favoured amounts of precursors for CO2 fixation pathways, e.g., the Calvin-Benson-Bassham (CBB) cycle. Furthermore, we demonstrated that formate could directly be utilized as a carbon source by yeast to synthesize endogenous amino acids. The engineered strain achieved a one-carbon (C1) assimilation efficiency of 9.2%, which was the highest efficiency observed in the co-utilization of 2G and 3G feedstocks. We applied this strategy for productions of both bulk and value-added chemicals, including ethanol, free fatty acids (FFAs), and longifolene, resulting in yield enhancements of 18.4%, 49.0%, and ~100%, respectively. The strategy demonstrated here for co-utilization of 2G and 3G feedstocks sheds lights on both basic and applied research for the up-coming establishment of 3G biorefineries.

     

    • FullText(HTML)
  • loading
    • References(61)
  • [1]
    W. Qiao; S. Xu; Z. Liu; X. Fu; H. Zhao; S. Shi, Bioresour. Technol. 364 (2022) 128095.
    [2]
    G. Hu; Z. Li; D. Ma; C. Ye; L. Zhang; C. Gao; L. Liu; X. Chen, Nat. Catal. 4 (2021) 395-406.
    [3]
    C.D. Scown, Trends Biotechnol. 40 (2022) 1415-1424.
    [4]
    P. Havlik; U.A. Schneider; E. Schmid; H. Bottcher; S. Fritz; R. Skalsky; K. Aoki; S.D. Cara; G. Kindermann; F. Kraxner; S. Leduc; I. Mccallum; A. Mosnier; T. Sauer; M. Obersteiner, Energy Policy 39 (2011) 5690-5702.
    [5]
    Z. Liu; K. Wang; Y. Chen; T. Tan; J. Nielsen, Nat. Catal. 3 (2020) 274-288.
    [6]
    J.C. Liao; L. Mi; S. Pontrelli; S. Luo, Nature reviews. Microbiology 14 (2016) 288-304.
    [7]
    Z. Liu; H. Li; X. Gao; X. Guo; S. Wang; Y. Fang; G. Song, Nat. Commun. 13 (2022) 4716.
    [8]
    D. Cai; P. Li; Z. Luo; P. Qin; C. Chen; Y. Wang; Z. Wang; T. Tan, Bioresour. Technol. 211 (2016) 117-124.
    [9]
    W. Deng; Y. Feng; J. Fu; H. Guo; Y. Guo; B. Han; Z. Jiang; L. Kong; C. Li; H. Liu; P.T.T. Nguyen; P. Ren; F. Wang; S. Wang; Y. Wang; Y. Wang; S.S. Wong; K. Yan; N. Yan; X. Yang; Y. Zhang; Z. Zhang; X. Zeng; H. Zhou, Green Energy Environ. 8 (2023) 10-114.
    [10]
    M. Wei; G. Li; H. Xie; W. Yang; H. Xu; S. Han; J. Wang; Y. Meng; Q. Xu; Y. Li; N. Chen; C. Zhang, Bioresour. Technol. 354 (2022) 127196.
    [11]
    R. Fujiwara; S. Noda; T. Tanaka; A. Kondo, Nat. Commun. (2020) 279.
    [12]
    M.T. Fernandez-Sandoval; J. Galindez-Mayer; F. Bolivar; G. Gosset; O.T. Ramirez; A. Martinez, Microb. Cell Fact. 18 (2019) 145.
    [13]
    R. Ledesma-Amaro; Z. Lazar; M. Rakicka; Z. Guo; F. Fouchard; A.-M.C.-L. Coq; J.-M. Nicaud, Metab. Eng. 38 (2016) 115-124.
    [14]
    W. Wei; P. Zhang; Y. Shang; Y. Zhou; B.C. Ye, Bioresour. Technol. 314 (2020) 123726.
    [15]
    N. Wei; E.J. Oh; G. Million; J.H. Cate; Y.S. Jin, ACS Synth. Biol. 4 (2015) 707-713.
    [16]
    Y.J. Lee; P. Hoang Nguyen Tran; J.K. Ko; G. Gong; Y. Um; S.O. Han; S.M. Lee, Front Bioeng. Biotechnol. 10 (2022) 826787.
    [17]
    S.B. Lee; M. Tremaine; M. Place; L. Liu; A. Pier; D.J. Krause; D. Xie; Y. Zhang; R. Landick; A.P. Gasch; C.T. Hittinger; T.K. Sato, Metab. Eng. 68 (2021) 119-130.
    [18]
    S. Watanabe; T. Kodaki; K. Makino, J. Biol. Chem. 280 (2005) 10340-10349.
    [19]
    S. Watanabe; A.A. Saleh; S.P. Pack; N. Annaluru; T. Kodaki; K. Makino, J. Biotechnol. 130 (2007) 316-319.
    [20]
    J.T. Cunha; P.O. Soares; A. Romani; J.M. Thevelein; L. Domingues, Biotechnol. Biofuels Bioprod. 12 (2019) 20.
    [21]
    J.H. Bae; M.J. Kim; B.H. Sung; Y.S. Jin; J.H. Sohn, Biotechnol. Biofuels Bioprod. 14 (2021) 223.
    [22]
    V. Endalur Gopinarayanan; N.U. Nair, Nat. Commun. 9 (2018) 1233.
    [23]
    X. Li; Y. Wang; G. Li; Q. Liu; R. Pereira; Y. Chen; J. Nielsen, Nat. Catal. 4 (2021) 783-796.
    [24]
    J. Shi; Y. Jiang; Z. Jiang; X. Wang; X. Wang; S. Zhang; P. Han; C. Yang, Chem. Soc. Rev. 44 (2015) 5981-6000.
    [25]
    J. Li; J. Ren; S. Li; G. Li; M.M.-J. Li; R. Li; Y.S. Kang; X. Zou; Y. Luo; B. Liu; Y. Zhao, Green Energy Environ. (2023), doi.org/10.1016/j.gee.2023.05.003.
    [26]
    H. Wang; Z. Liang; M. Tang; G. Chen; Y. Li; W. Chen; D. Lin; Z. Zhang; G. Zhou; J. Li; Z. Lu; K. Chan; T. Tan; Y. Cui, Joule 3 (2019) 1927-1936.
    [27]
    X. Tan; J. Nielsen, Chem. Soc. Rev. 51 (2022) 4763-4785.
    [28]
    M. Wang; Q. Zou; X. Dong; N. Xu; R. Shao; J. Ding; Y. Zhang; J. Qiao, Green Energy & Environ. 8 (2023) 893-903.
    [29]
    J. Li; Z. Zhang; W. Hu, Green Energy & Environ. 7 (2022) 855-857.
    [30]
    K. Wang; Y. Da; H. Bi; Y. Liu; B. Chen; M. Wang; Z. Liu; J. Nielsen; T. Tan, Renewable Energy 208 (2023) 331-340.
    [31]
    O. Yishai; S.N. Lindner; J. Gonzalez De La Cruz; H. Tenenboim; A. Bar-Even, Curr. Opin. Chem. Biol. 35 (2016) 1-9.
    [32]
    L. Calzadiaz-Ramirez; A.S. Meyer, Curr. Opin. Biotechnol. 73 (2022) 95-100.
    [33]
    S. Kim; S.N. Lindner; S. Aslan; O. Yishai; S. Wenk; K. Schann; A. Bar-Even, Nat. Chem. Biol. 16 (2020) 538-545.
    [34]
    O. Yishai; M. Bouzon; V. Doring; A. Bar-Even, ACS Synth. Biol. 7 (2018) 2023-2028.
    [35]
    J. Bang; C.H. Hwang; J.H. Ahn; J.A. Lee; S.Y. Lee, Nat. Microbiol. 5 (2020) 1459-1463.
    [36]
    R.A. Arkowitz; R.H. Abeles, Biochemistry 30 (1991) 4090-4097.
    [37]
    S. Gleizer; R. Ben-Nissan; Y.M. Bar-On; N. Antonovsky; E. Noor; Y. Zohar; G. Jona; E. Krieger; M. Shamshoum; A. Bar-Even; R. Milo, Cell 179 (2019) 1255-1263 e1212.
    [38]
    T. Gassler; M. Sauer; B. Gasser; M. Egermeier; C. Troyer; T. Causon; S. Hann; D. Mattanovich; M.G. Steiger, Nat. Biotechnol. 38 (2020) 210-216.
    [39]
    Y. Zhang; J. Zhou; Y. Zhang; T. Liu; X. Lu; D. Men; X.E. Zhang, ACS Synth. Biol. 10 (2021) 707-715.
    [40]
    B.M. Long; W.Y. Hee; R.E. Sharwood; B.D. Rae; S. Kaines; Y.L. Lim; N.D. Nguyen; B. Massey; S. Bala; S. Von Caemmerer; M.R. Badger; G.D. Price, Nat. Commun. 9 (2018) 3570.
    [41]
    J.H. Park; D.H. Kim; H.S. Kim; G.F. Wells; H.D. Park, Bioresour. Technol. 281 (2019) 318-325.
    [42]
    B. Endrodi; E. Kecsenovity; A. Samu; F. Darvas; R.V. Jones; V. Torok; A. Danyi; C. Janaky, ACS Energy Lett 4 (2019) 1770-1777.
    [43]
    F. Xia, J. Du, K. Wang, L. Liu, L. Ba, H. Liu, Y. Liu, 70 (2022) 11336-11343.
    [44]
    Y.J. Zhou; N.A. Buijs; Z. Zhu; J. Qin; V. Siewers; J. Nielsen, Nat. Commun. 7 (2016) 11709.
    [45]
    Y. Zhang; J. Wang; Z. Wang; Y. Zhang; S. Shi; J. Nielsen; Z. Liu, Nat. Commun. 10 (2019) 1053.
    [46]
    Y. Zhang; M. Su; N. Qin; J. Nielsen; Z. Liu, Microb. Cell Fact. 19 (2020) 226.
    [47]
    K. Wang; Y. Liu; Z. Wu; Y. Wu; H. Bi; Y. Liu; M. Wang; B. Chen; J. Nielsen; Z. Liu; T. Tan, Green Carbon 1 (2023) 65-74.
    [48]
    E. Fischer; N. Zamboni; U. Sauer, Anal. Biochem. 325 (2004) 308-316.
    [49]
    C. Su; L. Qi; D. Cai; B. Chen; H. Chen; C. Zhang; Z. Si; Z. Wang; G. Li; P. Qin, Renewable Energy 162 (2020) 1125-1131.
    [50]
    C. Su; C. Zhang; Y. Wu; Q. Zhu; J. Wen; Y. Wang; J. Zhao; Y. Liu; P. Qin; D. Cai, Renewable Energy 200 (2022) 592-600.
    [51]
    H. Bi; C. Xv; C. Su; P. Feng; C. Zhang; M. Wang; Y. Fang; T. Tan, Fermentation 8 (2022) 532.
    [52]
    S. Chen; Z. Xu; B. Ding; Y. Zhang; S. Liu; C. Cai; M. Li; B.E. Dale; M. Jin, Sci. Adv. 9 (2023) eadd8835.
    [53]
    T.K. Sato; M. Tremaine; L.S. Parreiras; A.S. Hebert; K.S. Myers; A.J. Higbee; M. Sardi; S.J. Mcilwain; I.M. Ong; R.J. Breuer; R. Avanasi Narasimhan; M.A. Mcgee; Q. Dickinson; A. La Reau; D. Xie; M. Tian; J.L. Reed; Y. Zhang; J.J. Coon; C.T. Hittinger; A.P. Gasch; R. Landick, PLoS Genet. 12 (2016) e1006372.
    [54]
    S.W. Jones; A.G. Fast; E.D. Carlson; C.A. Wiedel; J. Au; M.R. Antoniewicz; E.T. Papoutsakis; B.P. Tracy, Nat. Commun. 7 (2016) 12800.
    [55]
    Y.J. Li; M.M. Wang; Y.W. Chen; M. Wang; L.H. Fan; T.W. Tan, Sci. Rep. 7 (2017) 43875.
    [56]
    H. Moon; S. Kim; B.H. Jo; H.J. Cha, J. CO2 Util. 39 (2020) 101172.
    [57]
    C. Molina-Fernandez; P. Luis, J. CO2 Util. 47 (2021) 101475.
    [58]
    T.G. Cooper; H.G. Wood, J. Biol. Chem. 246 (1971) 5488-5490.
    [59]
    C. Zhan; X. Li; G. Lan; E.E.K. Baidoo; Y. Yang; Y. Liu; Y. Sun; S. Wang; Y. Wang; G. Wang; J. Nielsen; J.D. Keasling; Y. Chen; Z. Bai, Nat. Catal. 6 (2023) 435-450.
    [60]
    K. Paramasivan; S. Mutturi, Crit Rev Biotechnol 37 (2017) 974-989.
    [61]
    Y. Cao; R. Zhang; W. Liu; G. Zhao; W. Niu; J. Guo; M. Xian; H. Liu, Sci. Rep. 9 (2019) 95.
    • Relative Articles
    • Supplements(0)
    • Cited By
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索
    Get Citation
    PDF
    XML

    Article Metrics

    Article views (99) PDF downloads(11) Cited by()
    Proportional views

    Publish With GEE

    Contact

    • Email：gee@ipe.ac.cn
    • Tel：010-82627075
    • Address：North 2nd street, Zhongguancun, Haidian District, Beijing, China

    Links

    京ICP备10002620号-1京公网安备 11010802039050号

    Supported by: Beijing Renhe Information Technology Co. Ltd    

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return