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Yaoqiang Wang, Gang Xiao, Jianmin Xing, Haijia Su. Clostridium butyricum Mineralization of Intracellular Gold Nanoclusters to Boost Biohydrogen Production Using Visible Light. Green Energy&Environment. doi: 10.1016/j.gee.2025.12.008
Citation: Yaoqiang Wang, Gang Xiao, Jianmin Xing, Haijia Su. Clostridium butyricum Mineralization of Intracellular Gold Nanoclusters to Boost Biohydrogen Production Using Visible Light. Green Energy&Environment. doi: 10.1016/j.gee.2025.12.008
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Clostridium butyricum Mineralization of Intracellular Gold Nanoclusters to Boost Biohydrogen Production Using Visible Light

doi: 10.1016/j.gee.2025.12.008

  • a. State Key Laboratory of Green Biomanufacturing, Beijing Key Laboratory of Green Chemicals Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China;

  • b. CAS Key Laboratory of Green Process and Engineering & State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China;

  • c. Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, 100193, PR China

Funds:

This research was funded by the National Natural Science Foundation of China (22408378, 22408019) and the Fundamental Research Funds for the Central Universities (PY2517).

  • Received date 11 August 2025, Accepted date 11 December 2025, RevRecd date 29 October 2025, Available online 18 December 2025
    Abstract
  • Photocatalytic intracellular biohybrid systems hold great potential for enhancing biohydrogen production using solar energy. yet conventional approaches face nanoparticle toxicity and inefficient transmembrane electron transfer. Here, we developed an intracellular biohybrid system via in-situ biomineralization of gold nanoclusters (Au NCs, 2.15 ± 0.68 nm) within C. butyricum. This biosynthesis leveraged native ion-transport proteins for Au(III) uptake, synthesizing photocatalytically active Au NCs through cysteine-mediated reduction. This intracellular engineering confines photoexcitation and electron transfer within bacterial cells, eliminating transmembrane energy losses. As expected, the photoexcited Au NCs significantly enhanced intracellular NADH regeneration and ATP synthesis versus dark controls, boosting metabolic reducing power and energy. This resulted in a 70.42% increase in hydrogen production (2.34 mol H2·mol-1 glucose). Furthermore, Au NCs eliminated reactive oxygen species (ROS), improving system viability and stability under visible light. This work establishes a platform for engineering biocompatible hybrid systems via endogenous nanophotocatalyst self-assembly.

     

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    • References(64)
  • [1]
    T. Singh, A. Sehgal, R. Singh, S. Sharma, D.B. Pal, H.M. Tashkandi, R. Raddadi, S. Harakeh, S. Haque, M. Srivastava, Renewable Sustainable Energy Rev. 183 (2023) 113389.
    [2]
    S. Li, Q. Kang, J. Baeyens, H. Zhang, Y. Deng, Earth Environ. Sci. (2020), p 012011.
    [3]
    P. Nikolaidis, A. Poullikkas, Renewable Sustainable Energy Rev. 67 (2017) 597-611.
    [4]
    M. El-Qelish, G.K. Hassan, S. Leaper, P. Dessi, A. Abdel-Karim, J. Environ. Manage. 316 (2022) 115239.
    [5]
    F. Suleman, I. Dincer, M. Agelin-Chaab, Int. J. Hydrogen Energy 40 (2015) 6976-6987.
    [6]
    B.S. Rathi, P.S. Kumar, G. Rangasamy, S. Rajendran, Int. J. Hydrogen Energy 52 (2024) 115-138.
    [7]
    H. Ogata, K. Nishikawa, W. Lubitz, Nat. 520 (2015) 571-574.
    [8]
    K.A. Brown, M.B. Wilker, M. Boehm, G. Dukovic, P.W. King, J. Am. Chem. Soc. 134 (2012) 5627-5636.
    [9]
    B. Wang, C. Zeng, K.H. Chu, D. Wu, H.Y. Yip, L. Ye, P.K. Wong, Adv. Energy Mater. 7 (2017) 1700611.
    [10]
    D. Wu, W. Zhang, B. Fu, Z. Zhang, Joule 6 (2022) 2293-2303.
    [11]
    X.-M. Wang, L. Chen, R.-L. He, S. Cui, J. Li, X.-Z. Fu, Q.-Z. Wu, H.-Q. Liu, T.-Y. Huang, W.-W. Li, Nanoscale 14 (2022) 8409-8417.
    [12]
    Z. Jiang, B. Wang, C.Y. Jimmy, J. Wang, T. An, H. Zhao, H. Li, S. Yuan, P.K. Wong, Nano Energy 46 (2018) 234-240.
    [13]
    N. Kosem, Y. Honda, M. Watanabe, A. Takagaki, Z.P. Tehrani, F. Haydous, T. Lippert, T. Ishihara, Catal. Sci. Technol. 10 (2020) 4042-4052.
    [14]
    S. Cui, L.-J. Tian, J. Li, X.-M. Wang, H.-Q. Liu, X.-Z. Fu, R.-L. He, P.K. Lam, T.-Y. Huang, W.-W. Li, Chem. Eng. J. 428 (2022) 131254.
    [15]
    B. Luo, Y.Z. Wang, D. Li, H. Shen, L.X. Xu, Z. Fang, Z. Xia, J. Ren, W. Shi, Y.C. Yong, Adv. Energy Mater. 11 (2021) 2100256.
    [16]
    K.K. Sakimoto, A.B. Wong, P. Yang, Sci. 351 (2016) 74-77.
    [17]
    Z. Wang, D. Gao, H. Geng, C. Xing, J. Mater. Chem. A 9 (2021) 19788-19795.
    [18]
    H. Zhang, H. Liu, Z. Tian, D. Lu, Y. Yu, S. Cestellos-Blanco, K.K. Sakimoto, P. Yang, Nat. Nanotechnol. 13 (2018) 900-905.
    [19]
    Y. Wang, Y. Zhao, S. Wang, G. Xiao, Y. Jin, Z. Wang, H. Su, ACS Sustainable Chem. Eng. 1 (2023) 300- 311.
    [20]
    B.-X. Zheng, J.-N. Yuan, P. Su, X. Yan, Q. Chen, M. Yuan, F.-X. Xiao, J. Mater. Chem. A 13 (2025) 4908-4920.
    [21]
    G. Yang, Z. Wang, F. Du, F. Jiang, X. Yuan, J.Y. Ying, J. Am. Chem. Soc. 145 (2023) 11879-11898.
    [22]
    K. Liu, Z. Wang, Y. Xie, Y. Wei, W. Liu, T. Feng, Y. Liu, H. Zhu, X. Yuan, Chin. Chem. Lett. (2025) 111701.
    [23]
    Z.H. Zheng, F.X. Xiao, Y.J. Xu, Small 21 (2025) 2505282.
    [24]
    Z.Y. Li, M. Yuan, F.X. Xiao, Small 21 (2025) 2409513.
    [25]
    Y.-B. Li, F.-X. Xiao, Chem. Sci. 16 (2025) 2661-2672.
    [26]
    F. Mi, N. Zhao, L. Jin, Z. Zhang, X. Wang, X. Fang, W. Li, Z. Liu, P. Shu, X. Zhang, BMEMat 3 (2025) e12126.
    [27]
    Y.-B. Li, F.-X. Xiao, Inorg. Chem. 64 (2025) 3608-3615.
    [28]
    M. Kus-Liskiewicz, P. Fickers, I. Ben Tahar, Int. J. Mol. Sci. 22 (2021) 10952.
    [29]
    T.S. Dasari, Y. Zhang, H. Yu, Biochem. Pharmacol. 4 (2015) 199.
    [30]
    J. Depciuch, M. Stec, M. Kandler, J. Baran, M. Parlinska-Wojtan, Photodiagn. Photodyn. Ther. 30 (2020) 101670.
    [31]
    N. Kornienko, K.K. Sakimoto, D.M. Herlihy, S.C. Nguyen, A.P. Alivisatos, C.B. Harris, A. Schwartzberg, P. Yang, Proc. Natl. Acad. Sci. 113 (2016) 11750-11755.
    [32]
    H. An, Z. Song, P. Li, G. Wang, B. Ma, X. Wang, Appl. Nanosci. 10 (2020) 617-622.
    [33]
    E. Fontes, C. Ramos, C. Ottoni, R. De Souza, E. Antolini, A. Neto, Renewable Energy 167 (2021) 954-959.
    [34]
    Y. Negishi, K. Nobusada, T. Tsukuda, J. Am. Chem. Soc. 127 (2005) 5261-5270.
    [35]
    Y.S. Borghei, M. Hosseini, M.R. Ganjali, Sens. Actuators, B 273 (2018) 1618-1626.
    [36]
    W. Luo, C. Wang, J. Min, H. Luo, RSC Adv. 13 (2023) 7425-7431.
    [37]
    H. Shan, J. Shi, T. Chen, Y. Cao, Q. Yao, H. An, Z. Yang, Z. Wu, Z. Jiang, J. Xie, ACS nano 17 (2023) 2368-2377.
    [38]
    J. Qin, S. Wang, H. Ren, Y. Hou, X. Wang, Appl. Catal. B 179 (2015) 1-8.
    [39]
    B. Ma, S. Sun, H. He, R. Lv, J. Deng, T. Huo, Y. Zhao, H. Yu, L. Zhou, Ind. Eng. Chem. Res. 58 (2019) 23567-23573.
    [40]
    T. Anusha, K.S. Bhavani, J.S. Kumar, P.K. Brahman, R.Y. Hassan, Bioelectrochemistry 143 (2022) 107935.
    [41]
    Y. Li, W. Ho, K. Lv, B. Zhu, S.C. Lee, Appl. Surf. Sci. 430 (2018) 380-389.
    [42]
    J. Yan, W. Ye, X. Liang, S. Wang, J. Xie, K. Zhong, M. Bao, J. Yang, H. Wen, S. Li, Environ. Res. 183 (2020) 109157.
    [43]
    H. Xu, E. He, W.J. Peijnenburg, L. Song, L. Zhao, X. Xu, X. Cao, H. Qiu, J. Hazard. Mater. 415 (2021) 125616.
    [44]
    A. Rai, S. Seena, T. Gagliardi, P. Palma, Adv. Colloid Interface Sci. (2023) 102951.
    [45]
    S.M. Kalantarian, P. Slovensky, Z. Wang, V. Romanovski, E. Romanovskaia, M. Halama, M. Auinger, H.Y. Nie, Y.S. Hedberg, Part. Part. Syst. Charact. (2025) 70001.
    [46]
    H. Kim, Y.S. Hwang, M. Kim, S.B. Park, RSC Med. Chem. 12 (2021) 1037-1045.
    [47]
    X. Wang, T. Saba, H.H. Yiu, R.F. Howe, J.A. Anderson, J. Shi, Chem 2 (2017) 621-654.
    [48]
    J. Li, J. Ge, Z. Zhang, J. Qiang, T. Wei, Y. Chen, Z. Li, F. Wang, X. Chen, Sens. Actuators, B 296 (2019) 126578.
    [49]
    G. Cai, B. Jin, P. Monis, C. Saint, Biotechnol. Bioeng. 110 (2013) 338-342.
    [50]
    R. Lukajtis, I. Holowacz, K. Kucharska, M. Glinka, P. Rybarczyk, A. Przyjazny, M. Kaminski, Renewable Sustainable Energy Rev. 91 (2018) 665-694.
    [51]
    Y. Lin, J. Shi, W. Feng, J. Yue, Y. Luo, S. Chen, B. Yang, Y. Jiang, H. Hu, C. Zhou, Sci. Adv. 9 (2023) eadg5858.
    [52]
    M.C. Schoelmerich, V. Muller, Cell. Mol. Life Sci. 77 (2020) 1461-1481.
    [53]
    J. Lin, H. Zhang, Z. Chen, Y. Zheng, ACS nano 4 (2010) 5421-5429.
    [54]
    M. Hayyan, M.A. Hashim, I.M. Alnashef, Chem. Rev. 116 (2016) 3029-3085.
    [55]
    B. Santiago-Gonzalez, A. Monguzzi, J.M. Azpiroz, M. Prato, S. Erratico, M. Campione, R. Lorenzi, J. Pedrini, C. Santambrogio, Y. Torrente, Sci. 353 (2016) 571-575.
    [56]
    Y. Gao, N. Shao, Y. Pei, Z. Chen, X.C. Zeng, ACS nano 5 (2011) 7818-7829.
    [57]
    Y. Ueno, T. Kawai, S. Sato, S. Otsuka, M. Morimoto, J. Ferment. Bioeng. 79 (1995) 395-397.
    [58]
    J. Zhang, C. Fan, H. Zhang, Z. Wang, J. Zhang, M. Song, Int. J. Hydrogen Energy 43 (2018) 8729-8738.
    [59]
    G.K. Dinesh, R. Chauhan, S. Chakma, Renewable Sustainable Energy Rev. 92 (2018) 807-822.
    [60]
    I. Akkerman, M. Janssen, J. Rocha, R.H. Wijffels, Int. J. Hydrogen Energy 27 (2002) 1195-1208.
    [61]
    K.X. Lee, K. Shameli, Y.P. Yew, S.-Y. Teow, H. Jahangirian, R. Rafiee-Moghaddam, T.J. Webster, Int. J. Nanomed. (2020) 275-300.
    [62]
    X. Yu, H. Li, C. Xu, Z. Xu, S. Chen, W. Liu, T. Zhang, H. Sun, Y. Ge, Z. Qi, Adv. Sci. 11 (2024) 2400097.
    [63]
    M. Martins, C. Toste, I.A. Pereira, Angew. Chem., Int. Ed. 60 (2021) 9055-9062.
    [64]
    B. Ramprakash, A. Incharoensakdi, Int. J. Hydrogen Energy 45 (2020) 6254-6261.
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